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What media would we use to culture Campylobacter?
Modified charcoal cefoperazone desoxycholate agar (mCCD agar)
What proteins are used for Salmonella typing?
O ag on bacterial wall
H ag on flagella
What gene makes Salmonella tolerant to stomach acid?
Atr gene
Which Shigella species is the most common in the UK?
S. sonnei
If we see red colonies on XLD agar, what bacteria is present?
Shigella
If we see black colonies on XLD agar, what bacteria is present?
Salmonella
TCBS agar is used for what bacteria?
Vibrio cholera
How would we diagnose E. coli 0157
Molecular techniques (precise)
Sorbitol MacConkey agar (colourless colonies)
API 20E
latex agglutination test
Long-lasting immunity is associated with what virus?
Rotavirus
What protozoa is associated with swimming in lakes/rivers near fields?
Gardia lamblia
How do we detect eukaryotes?
Direct microscopy
concentration methods
staining
When and where do we see the most viral gastroentieritis?
Winter months
Often in children (schools, nurseries)
Shigella uses what protein to enter M cells?
Outer membrane protein IpaA
How does Salmonella enter host cell?
Attaches to receptors on M cells and apica cells
Type 3 secretory system injects bacterial protein into host cells which causes membrane ruffling
Bacteria uptake into a vacuole
Why do we have to be careful when treating patient with E. coli?
Antibitocs will trigger release of shigga toxin (patient will get worst)